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Introduction

 

 

The Department of Microbiology has started functioning in 1993. Presently  following sections are working

 

1.      BACTERIOLOGY

2.      MYCOBACTERIOLOGY

3.      SEROLOGY & IMMUNOLOGY

4.      MYCOLOGY

5.      PARASITOLOGY

6.      VIROLOGY

7.      MOLECULAR BIOLOGY

8.      HOSPITAL INFECTION CONTROL

 

BACTERIOLOGY:

 

Culture & Sensitivity testing of

  • Stool for Hanging drop , All Gram stains, ,Leptospaira for Dark field Microscopy & Stool for Ova & Cyst

  • All  Aerobic / Anaerobic Culture & Sensitivity (Urine ,Sputum ,Pus , body fluids, discharges, CSF, all type of Catheters  etc.)

  • Blood Culture & Sensitivity

  • Fungal Cultures (various Samples)

 

MYCOBACTERIOLOGY:  

  • AFB(Z.N) staining,

  • Mycobacterium(AFB)Cultures/ Culture & Sensitivity  for all types of  Samples

  • Mantoux test (PPD)

  • GeneXpert for MTB/RIF

 

SEROLOGY & IMMUNOLOGY:

  • Procalcitonin (PCT) , HBsAg, HIV(RVabs),&HCV Rapid tests

  • ASO TITRE, CRP test, R.A.FACTOR, VDRL test  (Blood &CSF)

  • WIDAL test (Quantitative)  & TPHA

Nephlometric method

  • ASO TITRE, CRP test, R.A.FACTOR

ELISA method

  • HBsAg , HIV(RVabs) & HCV by  ELISA

Special ELISA methods

  • Anti CCP ELISA

  • ANA IgM ELISA

  • dS DNA ELISA

  • Anti cardiolipin ELISA

  • Leptospira ELISA

  •  Scrub Typhus ELISA

  • Protein C & S by ELISA

  • H-Pylori IgG by ELISA

  • H-Pylori IgM by ELISA

  • Anti Intrinsic factor Antibody (AIFA) by ELISA

  • Anti Parietal cell Antibody (APCA) by ELISA

  • Anti  GBM  IgG by ELISA

  • Endomysium IgA by ELISA 

  • HBsAg titre by ELISA

RID methods

  • Complement (C 3) & Complement (C 4)

Western blot methods

  • ANA Profile

  • Anti pANCA/cANCA,

  • HIV (RV abs )

  • Neuronal Antigen profile-2

Immunofluoroscence

  • ANA IFA

 

MYCOLOGY:

  • Culture, Wet mount, KOH preparation, India Ink & Candida anti-fungal sensitivity

 

PARASITOLOGY :

 

Wet mount for Ova & Cyst, Modified Z-N staining cryptosporidium Isospora.

  • HCV RT PCR

  • M TB RT PCR

  • HSV I & II RT PCR

  • Leptospira RT PCR

  • HBV RT PCR

  • HIV RT PCR

 

 

VIROLOGY :

 

Elisa Tests

  • Dengue NS1-Ag

  • Dengue  IgM

  • Hepatitis A virus IgM

  • Hepatitis B virussurface antigen

  • Hepatitis C virus  Total Antibodies

  • Hepatitis E virus IgM

  • Measles IgM

  • Mumps IgM

  • Rubella IgM

  • Japanese encephalitis virus IgM

  • Chikungunya virus IgM

  • Rotavirus  Antigen

  • Epstein–Barr virus IgM

  • Cytomegalovirus IgM

  • Parvovirus B19 IgM

Molecular biology tests

  • Herpes simplex virus I Nested PCR

  • Influenza A (H1N1)/pan09Real Time RT-PCR

  • Respiratory syncytial virusReal Time RT-PCR

  • ZikavirusReal Time RT-PCR

  •  

MOLECULAR BIOLOGY :

 

  • PCR :HIV, HCV, HSV, HBV,  MTB

  •  

HOSPITAL INFECTION CONTROL :

 

1.   Hospital Surveillance for nosocomial infections for SVIMS,BIRRD, Hospital.(VAP, CAUTI, SSI, CLABSI)

2.   OT Surveillance

3.   Microbiological testings of Water, Dialysis samples

4.   Audits – Hand Hygiene audit, Biomedical Waste audit, Personal Protective Equipment audit, Surgical Prophylaxis audit

5.   Multi Drug Resistance Organisms Surveillance

 

 

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