Introduction

 

 

The Department of Microbiology has started functioning in 1993. Presently  following sections are working

 

1.      BACTERIOLOGY

2.      MYCOBACTERIOLOGY

3.      SEROLOGY & IMMUNOLOGY

4.      MYCOLOGY

5.      PARASITOLOGY

6.      VIROLOGY

7.      MOLECULAR BIOLOGY

8.      HOSPITAL INFECTION CONTROL

 

BACTERIOLOGY:

 

Culture & Sensitivity testing of

• Stool for Hanging drop , All Gram stains, ,Leptospaira for Dark field Microscopy & Stool for Ova & Cyst

• All  Aerobic / Anaerobic Culture & Sensitivity (Urine ,Sputum ,Pus , body fluids, discharges, CSF, all type of Catheters  etc.)

• Blood Culture & Sensitivity

• Fungal Cultures (various Samples)

 

MYCOBACTERIOLOGY:  

• AFB(Z.N) staining,

• Mycobacterium(AFB)Cultures/ Culture & Sensitivity  for all types of  Samples

• Mantoux test (PPD)

• GeneXpert for MTB/RIF

 

SEROLOGY & IMMUNOLOGY:

• Procalcitonin (PCT) , HBsAg, HIV(RVabs),&HCV Rapid tests

• ASO TITRE, CRP test, R.A.FACTOR, VDRL test  (Blood &CSF)

• WIDAL test (Quantitative)  & TPHA

Nephlometric method

• ASO TITRE, CRP test, R.A.FACTOR

ELISA method

• HBsAg , HIV(RVabs) & HCV by  ELISA

Special ELISA methods

• Anti CCP ELISA

• ANA IgM ELISA

• dS DNA ELISA

• Anti cardiolipin ELISA

• Leptospira ELISA

•  Scrub Typhus ELISA

• Protein C & S by ELISA

• H-Pylori IgG by ELISA

• H-Pylori IgM by ELISA

• Anti Intrinsic factor Antibody (AIFA) by ELISA

• Anti Parietal cell Antibody (APCA) by ELISA

• Anti  GBM  IgG by ELISA

• Endomysium IgA by ELISA 

• HBsAg titre by ELISA

RID methods

• Complement (C 3) & Complement (C 4)

Western blot methods

• ANA Profile

• Anti pANCA/cANCA,

• HIV (RV abs )

• Neuronal Antigen profile-2

Immunofluoroscence

• ANA IFA

 

MYCOLOGY:

• Culture, Wet mount, KOH preparation, India Ink & Candida anti-fungal sensitivity

 

PARASITOLOGY :

 

Wet mount for Ova & Cyst, Modified Z-N staining cryptosporidium Isospora.

• HCV RT PCR

• M TB RT PCR

• HSV I & II RT PCR

• Leptospira RT PCR

• HBV RT PCR

• HIV RT PCR

 

 

VIROLOGY :

 

Elisa Tests

• Dengue NS1-Ag

• Dengue  IgM

• Hepatitis A virus IgM

• Hepatitis B virussurface antigen

• Hepatitis C virus  Total Antibodies

• Hepatitis E virus IgM

• Measles IgM

• Mumps IgM

• Rubella IgM

• Japanese encephalitis virus IgM

• Chikungunya virus IgM

• Rotavirus  Antigen

• Epstein–Barr virus IgM

• Cytomegalovirus IgM

• Parvovirus B19 IgM

Molecular biology tests

• Herpes simplex virus I Nested PCR

• Influenza A (H1N1)/pan09Real Time RT-PCR

• Respiratory syncytial virusReal Time RT-PCR

• ZikavirusReal Time RT-PCR

•  

MOLECULAR BIOLOGY :

 

• PCR :HIV, HCV, HSV, HBV,  MTB

•  

HOSPITAL INFECTION CONTROL :

 

1.   Hospital Surveillance for nosocomial infections for SVIMS,BIRRD, Hospital.(VAP, CAUTI, SSI, CLABSI)

2.   OT Surveillance

3.   Microbiological testings of Water, Dialysis samples

4.   Audits – Hand Hygiene audit, Biomedical Waste audit, Personal Protective Equipment audit, Surgical Prophylaxis audit

5.   Multi Drug Resistance Organisms Surveillance