The Department is proud to be associated with human adult stem cells. For the first we have isolated CD34+human stem cells from the peripheral blood. The patients were g/Kg/day G-CSF and thus, mobilized stem cells from bone marrow intomgiven 5 blood were isolated using Fresenius ASTec204 cell separator and using Rvy kit. These stem cells were evaluated and transplanted into the heart both intra-coronary route in a group of 17 patients. All the patients showed remarkable improvement and were successfully doing their routine work very happily.
For the first time in India we have developed cardiomyocytes from the cultured human adult stem cells the work was published as a peer reviewed article in one of the acclaimed leading heart journal. The cradiogenesis photo graph appeared on the cover page of the journal.
1. P.V.G.K.SARMA and G.SUBRAMANYAM (2008). In vitro cardiogenesis can be initiated in Human CD34+ve cells. Indian Heart J (Original and Peer Reviewed Article) 60: 95-100.
MICROBIAL PATHOGENESIS:
The department has successfully cloned, sequenced and characterized UMPkinase, TMPkinase, TK, SHMT, isocitrate dehydrogenase, alpha amylase and Protein Tyrosine kinase genes from Staphylococcus aureus and the sequences have been deposited at gene bank (www.ncbi.nlm.nih.gov). The department is concentrating to identify specific drug targets for the development of novel and specific drugs against these Staphylococcus aureus infections. The concept being development of narrow and spectrum drugs rather than broad spectrum antibiotics which would minimize transfer of drug resistance and specifically help in curing the infections caused by Staphylococcus aureus.
Recently, Mr O. Hari Prasad has successfully submitted his Ph.D thesis ?Molecular characterization of UMP kinase and Thymidine monophosphate kinase from Staphylococcus aures ATCC 12600? to the SVIMS University and is waiting for the final viva voce.
FUNCTIONAL GENOMICS:
In the functional genomics area we have established:
1. ACE gene polymorphism in the patients suffering from type 2 diabetes and nephropathy
2. Deletion- insertion mutations in the exons 40 and 45 of human PKD1 gene
3. A new mutation in the exon 10 of human factor V gene
4. We have shown the relationship of intestinal alkaline phosphatase and the duodenal cancer caused by Helicobacter pylori
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